WITNESS® FeLV-FIV test is indicated for use when history and/or clinical signs may suggest an infection by feline retroviruses. It will also be particularly recommended prior to an FeLV vaccination, especially in cats belonging to an at risk population.
WITNESS® FeLV-FIV is a simple test based on Rapid Immuno Migration (RIMTM) technology.
FeLV antigen is detected using antibodies directed against the circulating p27 capside protein.
The detection of FIV is based on the presence of antibodies against the transmembrane region of the virus, using a synthetic peptide from the gp40 protein.
In both cases, sensitised colloidal gold particles will form a complex with either the p27 antigen (FeLV) or antibodies (FIV) present in the sample.
The formed complexes migrate along the strips. The complexes are then captured on a sensitised reaction line where their accumulation causes the formation of a clearly visible pink band.
Control bands located at the end of the reading windows (3) ensure that the test was performed correctly.
• The test can be performed on unclotted whole blood anticoagulated with EDTA or heparin, serum or plasma.
• Samples should always be collected with a sterile needle and syringe.
• Haemolysis does not significantly interfere with the test, but strongly haemolysed samples may partly obscure a weak positive band.
Anticoagulated whole blood samples should preferably be tested immediately after collection but not longer than 4 hours after collection, if stored at room temperature.
If testing is further delayed, samples should be kept refrigerated (+2°C and 8°C) for up to 4 days.
For prolonged storage, samples (serum and plasma only) should be kept frozen (-20°C).
A. 21/05/1o pouches, each containing 1 test device and desiccant
B. 1 buffer dropper bottle (of 2.8 ml).
C. Instructions for use.
D. 2/5/10 pipettes