INDICATION
Serological diagnosis of brucellosis (caused by Brucella melitensis, abortus and suis) by rapid slide agglutination.
ADMINISTRATION
Materials
- Automatic pipette with changeable pipette tips able to distribute a volume of 25 —30 pl.
- Dropper calibrated at 30 pl to facilitate antigen distribution (a calibrated dropper is enclosed in the BENGATEST pack). REMARK: If using a different sample volume than 30 pl, we recommend the use of an automatic pipette.
Reagents
Sera and antigen stored between +2°C and +8°C are placed at room temperature for 30 to 60 minutes before use.
Control
Include one positive and one negative serum in the sero-agglutination series as a control for antigen activity.
Procedure
It is essential to use two equal volumes of serum and buffered antigen to perform the test.
- Place a 25-30 pl drop of each serum under test onto the plate.
- Shake the vial of antigen gently and put an equal volume beside each of the sera. You may use the calibrated dropper enclosed in the BENGATEST for the distribution of 30 pl. We recommend the adherence to Good Laboratory Practices when placing the calibrated dropper.
- Carefully blend the antigen and the serum.
- Shake slowly at room temperature and read results 4 minutes later.
Result
- No agglutination: absence of antibodies
- Agglutination (even slight): presence of antibodies.
PACKING: 100 ml
